Interspecies Developmental Differences in Metabonomic Phenotypes of Lycium ruthenicum and L. barbarum Fruits

• DOI: 10.1021/acs.jproteome.8b00349
• Publication Date: 2018-08-07T16:26:20Z
• Authors: Qi Wang; Shaohua Zeng; Xiangyu Wu; Hehua Lei; Ying Wang; Huiru Tang
• Keywords: Lycium

Fruits of Lycium ruthenicum (LR) and L. barbarum (LB) in Solanaceae family contain abundant bioactive metabolites used widely as functional food natural medicine. To characterize the fruit developmental molecular phenotypes, we comprehensively analyzed metabolite composition both fruits at three stages using combined NMR, liquid chromatography–tandem mass spectrometry, gas chromatography-flame ionization detector/mass spectrometry methods. The metabonomes these were dominated by over 90 including sugars, amino acids, tricarboxylic acid (TCA) cycle intermediates, fatty choline metabolites, shikimate-mediated plant secondary metabolites. Metabolic phenotypes two species differed significantly all stages; LB contained more sugars acids but less TCA than LR. Interspecies differences for levels much greater after color-breaking precolor-breaking. Furthermore, LR osmolytes indicating different osmoregulation requirements during development. Significant also present biosynthesis LB. These findings provided essential metabolic information physiology their utilizations demonstrating usefulness this metabonomic phenotyping approach studying fundamental biochemistry