In Vivo Proteome of Pseudomonas aeruginosa in Airways of Cystic Fibrosis Patients

• DOI: 10.1021/acs.jproteome.9b00122
• Publication Date: 2019-05-07T08:50:36Z
• Authors: Xia Wu; Richard J. Siehnel; Jayanthi Garudathri; Benjamin J. Staud...; Katherine B. Hisert; Egon A. Ozer; Alan R. Hauser; Jimmy K. Eng; Colin Manoil; Pradeep K. Singh; James E. Bruce
• Keywords: Proteome; Ex vivo

Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on physiology patient airways and better understand how vivo bacterial functioning differs from vitro conditions, we investigated the proteomes 35 sputum samples 11 patients. We developed novel bacterial-enrichment method that relies differential centrifugation detergent treatment to enrich for bacteria improve identification proteome samples. Using two nonredundant peptides as cutoff, total 1304 proteins were identified directly compared ex vivo-grown populations same sample. Label-free quantitation comparison revealed up-regulation siderophore TonB-dependent receptors, remodeling central carbon metabolism including glyoxylate cycle lactate utilization, alginate overproduction. Knowledge these differences or others derived using presented methodology could lead future strategies aimed at altering compromise infectivity antibiotic efficacy.