Bidirectional Peptide Translocation through Ultrasmall Solid-State Nanopores

• DOI: 10.1021/acs.langmuir.4c03212
• Publication Date: 2024-09-20T08:09:26Z
• Authors: Guanghao Wei; Rui Hu; Wenlong Lu; Zhan Wang; Qing Zhao
• Keywords: Nanopore

It is important to obtain the configuration of polypeptides and sequence information on amino acids for understanding various life processes many biological applications. Nanopores, as a newly developed single-molecule detection technology, exhibit unique advantages in real-time dynamics detection. Here, we designed special peptide chain with 10 arginine head achieved successful by ultrasmall solid-state nanopores (2-3 nm). Unique bidirectional translocation signals were observed explained under framework charge distribution interaction nanopore wall. Two natural chains, histatin-5 angiopep-2, also explored experiments confirm our conjecture. Our could realize multiple detections same chain, offering possibilities high-resolution fingerprinting future.