Interaction of DNA with Cationic Lipid Mixtures—Investigation at Langmuir Lipid Monolayers

• DOI: 10.1021/acs.langmuir.7b02014
• Publication Date: 2017-09-05T22:47:54Z
• Authors: Christopher Janich; André Hädicke; Udo Bakowsky; Gerald Brezesinski; Christian Wölk
• Keywords: Cationic polymerization

Four different binary lipid mixtures composed of a cationic and the zwitterionic colipids DOPE or DPPC, which show DNA transfer activities in cell culture models, were investigated at soft air/water interface to identify transfection efficiency determining characteristics. Langmuir films are useful models investigate interaction between two-dimensional model system by using surface sensitive techniques, namely, epifluorescence microscopy infrared reflection-absorption spectroscopy. Especially, effect adsorbed on properties has been examined. Distinct differences composites found caused mixtures. containing form fluid monolayers with uniform distribution fluorescent probe presence absence physiologically relevant pressures. Only high nonphysiological pressures, monolayer collapses phase separation was observed if present subphase. In contrast, DPPC domains liquid condensed state The adsorption positively charged mixed induces is expressed morphology point appearance these domains.