pH-Dependent Adsorption Release of Doxorubicin on MamC-Biomimetic Magnetite Nanoparticles

• DOI: 10.1021/acs.langmuir.8b03109
• Publication Date: 2018-11-05T15:03:05Z
• Authors: Germán García Rubia; Ana Peigneux; Ylenia Jabalera; Javier Puerma; Francesca Oltolina; Kerstin Elert; Donato Colangelo; Jaime Gómez Morales; Maria Prat; Concepcion Jimene...
• Keywords: Nanocarriers; Surface Modification; Zeta potential

New biomimetic magnetite nanoparticles (hereafter BMNPs) with sizes larger than most common superparamagnetic were produced in the presence of recombinant MamC protein from Magnetococcus marinus MC-1 and functionalized doxorubicin (DOXO) intended as potential drug nanocarriers. Unlike inorganic nanoparticles, BMNPs controls their size morphology, providing them magnetic properties consistent a large moment per particle; moreover, it provides novel surface properties. display isoelectric point at pH 4.4, being strongly negatively charged physiological (pH 7.4). This allows both (i) functionalization DOXO, which is positively 7.4, (ii) stability DOXO–surface bond DOXO release to be dependent governed by electrostatic interactions. adsorption follows Langmuir–Freundlich model, coupling (binary nanoparticles) very stable (maximum 5% adsorbed). Conversely, when decreases, these interactions weaken, 5, released up ∼35% amount initially adsorbed. The DOXO–BMNPs cytotoxicity on GTL-16 human gastric carcinoma cell line dose-dependent manner, reaching about ∼70% mortality maximum tested, while nonloaded are fully cytocompatible. present data suggest that could useful nanocarriers adsorption-release changes local values.