Microparticles-in-Thermoresponsive/Bioadhesive Hydrogels as a Novel Integrated Platform for Effective Intra-articular Delivery of Triamcinolone Acetonide

Male Drug Carriers Polymers Viscosity Temperature Hydrogels 02 engineering and technology Triamcinolone Acetonide 01 natural sciences Polyethylene Glycols Rats 0104 chemical sciences Arthritis, Rheumatoid Spectroscopy, Fourier Transform Infrared Microscopy, Electron, Scanning Animals 0210 nano-technology
DOI: 10.1021/acs.molpharmaceut.0c00126 Publication Date: 2020-04-09T19:39:00Z
ABSTRACT
Intra-articular (IA) injection of thermoresponsive hydrogels coupled with microparticles (MPs) possess the benefit sustaining anti-inflammatory drug effect within joint cavity for rheumatoid arthritis treatment. Star-shaped poly(polyethylene glycol) methacrylate [Poly(PEGMA)] copolymers were synthesized using free radical polymerization technique and fully characterized. Triamcinolone acetonide (TA)-loaded PLA/mPEG–PDL MPs, previously optimized, integrated into copolymer solutions at various concentrations tested their gelation temperatures. The MPs-in-hydrogel formulations characterized scanning electron microscope (SEM), viscosity measurements, ex vivo bioadhesion, in vitro release studies. systems was assessed adjuvant-induced monoarthritic rat knee joints compared to Kenacort TA-loaded MPs. Two successfully synthesized; G-1 = poly(PEGMA188-ME-co-PEGMA475-ME) G-2 poly(PEGMA246-EE-co-PEGMA475-ME). Using tube inversion technique, gel formation found dependent on concentration. An irreversible aggregation obtained ≤10% (w/v), while a formed 20 30% (w/v) both upon increasing temperature. MP-hydrogel optimized temperatures 33 37 °C, respectively. SEM images revealed porous microstructures adsorption MP surfaces. formulas showed pseudoplastic behaviors, bioadhesion study confirmed bioadhesiveness excised cartilage. sustainment from MPs-hydrogels In studies proved superiority MP-in-hydrogels treatment induced arthritis, relative MPs alone, suggesting applicability this platform IA delivery.
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