Self-Interactions of Two Monoclonal Antibodies: Small-Angle X-ray Scattering, Light Scattering, and Coarse-Grained Modeling

• DOI: 10.1021/acs.molpharmaceut.1c00627
• Publication Date: 2021-12-23T13:54:40Z
• Authors: Sujata Mahapatra; Marco Polimeni; Lorenzo Gentiluomo; Dierk Roessner; Wolfgang Frieß; Günther H. J. Peters; Werner W. Streicher; Mikael Lund; Pernille Harris
• Keywords: 0303 health sciences; 03 medical and health sciences; X-Ray Diffraction; X-Rays; Scattering, Small Angle; Antibodies, Monoclonal; Monte Carlo Method; 3. Good health

Using light scattering (LS), small-angle X-ray scattering (SAXS), and coarse-grained Monte Carlo (MC) simulations, we studied the self-interactions of two monoclonal antibodies (mAbs), PPI03 and PPI13. With LS measurements, we obtained the osmotic second virial coefficient, B22, and the molecular weight, Mw, of the two mAbs, while with SAXS measurements, we studied the mAbs' self-interaction behavior in the high protein concentration regime up to 125 g/L. Through SAXS-derived coarse-grained representations of the mAbs, we performed MC simulations with either a one-protein or a two-protein model to predict B22. By comparing simulation and experimental results, we validated our models and obtained insights into the mAbs' self-interaction properties, highlighting the role of both ion binding and charged patches on the mAb surfaces. Our models provide useful information about mAbs' self-interaction properties and can assist the screening of conditions driving to colloidal stability.