Octreotide-Mediated Tumor-Targeted Drug Delivery via a Cleavable Doxorubicin–Peptide Conjugate

• DOI: 10.1021/acs.molpharmaceut.5b00487
• Publication Date: 2015-11-02T17:56:09Z
• Authors: Marco Lelle; Stefka Kaloyanova; Christoph Freidel; Marily Theodoropo...; Michael Musheev; Christof Niehrs; Günter Stalla; Kalina Peneva
• Keywords: 0301 basic medicine; Drug Carriers; Cell Survival; Antineoplastic Agents; Octreotide; 3. Good health; 03 medical and health sciences; Drug Delivery Systems; Doxorubicin; Cell Line, Tumor; Humans; Receptors, Somatostatin; Peptides

Although recent methods for targeted drug delivery have addressed many of the existing problems of cancer therapy associated with undesirable side effects, significant challenges remain that have to be met before they find significant clinical relevance. One such area is the delicate chemical bond that is applied to connect a cytotoxic drug with targeting moieties like antibodies or peptides. Here we describe a novel platform that can be utilized for the preparation of drug-carrier conjugates in a site-specific manner, which provides excellent versatility and enables triggered release inside cancer cells. Its key feature is a cleavable doxorubicin-octreotide bioconjugate that targets overexpressed somatostatin receptors on tumor cells, where the coupling between the two components was achieved through the first cleavable disulfide-intercalating linker. The tumor targeting ability and suppression of adrenocorticotropic hormone secretion in AtT-20 cells by both octreotide and the doxorubicin hybrid were determined via a specific radioimmunoassay. Both substances reduced the hormone secretion to a similar extent, which demonstrated that the tumor homing peptide is able to interact with the relevant cell surface receptors after the attachment of the drug. Effective drug release was quickly accomplished in the presence of the physiological reducing agent glutathione. We also demonstrate the relevance of this scaffold in biological context in cytotoxicity assays with pituitary, pancreatic, and breast cancer cell lines.