Biocompatible ELR-Based Polyplexes Coated with MUC1 Specific Aptamers and Targeted for Breast Cancer Gene Therapy

• DOI: 10.1021/acs.molpharmaceut.5b00712
• Publication Date: 2016-01-27T16:24:02Z
• Authors: Maria J. Piña; Alessandra Girotti; Mercedes Santos; J. Carlos Rodrígu...; F. Javier Arias
• Keywords: Aptamer; Zeta potential; MUC1

The search for new and biocompatible materials with high potential improvement is a challenge in gene delivery applications. A cell type specific vector made of elastin-like recombinamer (ELR) aptamers has been specifically designed the intracellular therapeutic material breast cancer therapy. lysine-enriched ELR was constructed complexed plasmid DNA to give positively charged stable polyplexes. Physical characterization these polyplexes showed particle size around 140 nm zeta approximately +40 mV. incorporation MUC1-specific into resulted slight decrease but increased transfection specificity MCF-7 cells respect MUC1-negative tumor line. After showing ability this aptamer-ELR which facilitated mainly by macropinocytosis uptake, we demonstrated its application suicide therapy using containing toxin PAP-S. strategy developed work about as polymeric supplier deliver MUC1-positive shows promising continues paving way ELRs biomedical field.