Enhanced Antiglioblastoma Efficacy of Neovasculature and Glioma Cells Dual Targeted Nanoparticles

Male Mice, Inbred BALB C 0303 health sciences Paclitaxel Polyesters Mice, Nude Glioma Antineoplastic Agents, Phytogenic Polyethylene Glycols Rats 3. Good health Mice 03 medical and health sciences Drug Delivery Systems Cell Line, Tumor Human Umbilical Vein Endothelial Cells Animals Humans Nanoparticles Glioblastoma
DOI: 10.1021/acs.molpharmaceut.6b00523 Publication Date: 2016-09-16T19:18:32Z
ABSTRACT
Combining treatment of anticancer cells and antiangiogenesis is considered to be a potential targeted strategy for brain glioblastoma therapy. In this study, by utilizing the overexpression Interleukin 13 receptor α2 (IL-13Rα2) on glioma heparan sulfate neovascular endothelial cells, we developed paclitaxel (PTX) loaded Pep-1 CGKRK peptide-modified PEG–PLGA nanoparticle (PC-NP-PTX) neovasculature dual-targeted chemotherapy enhance antiglioma efficacy. There were significant differences both enhancement cellular uptake in HUVEC C6 improvement vitro activity respect proliferation, tumor spheroid growth, tube formation, migration between PC-NP-PTX Taxol NP-PTX. As IC50 3.59 ± 0.056, 2.37 0.044, 1.38 0.028, 1.82 0.035, 1.00 0.016 μg/mL Taxol, NP-PTX, Pep-NP-PTX, CGKRK-NP-PTX, PC-NP-PTX, 0.44 0.006, 0.33 0.005, 0.25 0.19 0.004, 0.16 0.004 respectively. vivo distribution assays confirmed that accumulated effectively at site. showed longer median survival time 61 days when compared with (22 days), NP-PTX (24 Pep-NP-PTX (32 CGKRK-NP-PTX (34 days). The efficacy safety evaluation significantly enhanced displayed negligible acute toxicity.
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