Nanofiber Dressings Topically Delivering Molecularly Engineered Human Cathelicidin Peptides for the Treatment of Biofilms in Chronic Wounds

• DOI: 10.1021/acs.molpharmaceut.8b01345
• Publication Date: 2019-03-27T22:33:47Z
• Authors: Yajuan Su; Hongjun Wang; Biswajit Mishra; Jayaram Lakshmaia...; Jiang Jiang; Debra A. Reilly; Ronald R. Hollins; Mark A. Carlson; Guangshun Wang; Jingwei Xie
• Keywords: Acinetobacter baumannii; Cathelicidin; Chronic wound; Debridement (dental)

Biofilms of multidrug-resistant bacteria in chronic wounds pose a great challenge wound care. Herein, we report the topical delivery molecularly engineered antimicrobial peptides using electrospun nanofiber dressings as carrier for treatment biofilms diabetic wounds. Molecularly human cathelicidin peptide 17BIPHE2 was successfully encapsulated core pluronic F127/17BIPHE2-PCL core–shell nanofibers. The vitro release profiles showed an initial burst followed by sustained over 4 weeks. formulations effectively killed methicillin-resistant Staphylococcus aureus (MRSA) USA300. Similarly, containing nanofibers could also kill other including Klebsiella pneumoniae (104 to 106 CFU) and Acinetobacter baumannii 107 clinical strains without showing evident cytotoxicity skin cells monocytes. Importantly, 17BIPHE2-containing debridement caused five-magnitude decreases MRSA USA300 CFU biofilm-containing model based on type II mice. In combination with debridement, completely eliminate biofilms, providing one possible solution treatment. Taken together, biodegradable nanofiber-based developed this study can be utilized deliver treat