Ultrahigh-Speed Imaging of Rotational Diffusion on a Lipid Bilayer

• DOI: 10.1021/acs.nanolett.0c02516
• Publication Date: 2020-07-27T04:04:17Z
• Authors: Mahdi Mazaheri; Jens Ehrig; Alexey Shkarin; Vasily Zaburdaev; Vahid Sandoghdar
• Keywords: Rotational diffusion

We studied the rotational and translational diffusion of a single gold nanorod linked to supported lipid bilayer with ultrahigh temporal resolution two microseconds. By using home-built polarization-sensitive dark-field microscope, we recorded particle trajectories lateral precision 3 nm 4°. The large number trajectory points in our measurements allows us characterize statistics unprecedented detail. Our data show apparent signatures anomalous such as sublinear scaling mean-squared angular displacement negative values correlation function at small lag times. However, careful analysis reveals that these effects stem from residual noise contributions confirms normal diffusion. experimental approach observations can be extended investigate diffusive processes anisotropic nanoparticles other fundamental systems cellular membranes or two-dimensional fluids.