Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair

• DOI: 10.1021/acs.nanolett.2c02259
• Publication Date: 2022-07-25T12:52:47Z
• Authors: Min Han; Hongru Yang; Xiangdong Lu; Yuming Li; Zihao Liu; Feng Li; Zehan Shang; Xiaofeng Wang; Xuze Li; Junliang Li; Hong Liu; Tao Xin
• Keywords: Exosome

Exosomes derived from mesenchymal stem cells (MSCs) have been proven to exhibit great potentials in spinal cord injury (SCI) therapy. However, conventional two-dimensional (2D) culture will inevitably lead the loss of stemness MSCs, which substantially limits therapeutic potency MSCs exosomes (2D-Exo). three-dimensional (3D-Exo) possess higher efficiency wide applications Typically, exosome therapy that relies on local repeated injection results secondary and low efficiency. It is urgent develop a more reliable, convenient, effective delivery method achieve constant situ release. Herein, we proposed controlled 3D-exohydrogel hybrid microneedle array patch SCI repair situ. Our studies suggested with 3D-culturing could maintain their stemness, consequently, 3D-Exo effectively reduced SCI-induced inflammation glial scarring. Thus, it promising strategy for treatment SCI.