DNA-Templated Anchoring of Proteins for Programmable Cell Functionalization and Immunological Response

• DOI: 10.1021/acs.nanolett.2c03928
• Publication Date: 2022-12-28T20:05:32Z
• Authors: Mengyi Xiong; Gezhi Kong; Qin Liu; Lu Liu; Yao Yin; Ying Liu; Hui Yuan; Xiao-Bing Zhang; Weihong Tan
• Keywords: Immunological synapse; Surface Modification; Protein Engineering; Transduction (biophysics)

Membrane protein engineering exhibits great potential for cell functionalization. Although genetic strategies are sophisticated membrane engineering, there still exist some issues, including transgene insertional mutagenesis, laborious, complicated procedures, and low tunability. Herein, we report a DNA-templated anchoring of exogenous proteins on living membranes to realize programmable functionalization cells. Using DNA as scaffold, the model readily modified with proteins, which density ratio well their interactions can be precisely controlled through predictable hybridization. Then, natural killer (NK) cells were engineered gain ability eliminate immune checkpoint signaling at NK-tumor synapse, remarkably promoted NK activation in immunotherapy. Given versatile functions flexible designs DNA, this method has facilitate membrane-protein-based therapy.