Ultrathin and Flexible Bioelectronic Arrays for Functional Measurement of iPSC-Cardiomyocytes under Cardiotropic Drug Administration and Controlled Microenvironments

• DOI: 10.1021/acs.nanolett.3c00017
• Publication Date: 2023-03-09T17:45:54Z
• Authors: Wenkun Dou; Abdelkader Daoud; Xin Chen; Tiancong Wang; Manpreet Malhi; Zheyuan Gong; Fatemeh Mirshafiei; Min Zhu; Guanqiao Shan; Xi Huang; Jason T. Maynes; Yu Sun
• Keywords: Contractility; Multielectrode array

Emerging heart-on-a-chip technology is a promising tool to establish in vitro cardiac models for therapeutic testing and disease modeling. However, due the technical complexity of integrating cell culture chambers, biosensors, bioreactors into single entity, microphysiological system capable reproducing controlled microenvironmental cues regulate phenotypes, promote iPS-cardiomyocyte maturity, simultaneously measure dynamic changes cardiomyocyte function situ not available. This paper reports an ultrathin flexible bioelectronic array platform 24-well format higher-throughput contractility measurement under candidate drug administration or defined conditions. In array, carbon black (CB)-PDMS strain sensors were embedded detecting iPSC-CM signals. Carbon fiber electrodes pneumatic air channels integrated provide electrical mechanical stimulation improve maturation. Performed experiments validate that accurately reveals effects cardiotropic drugs identifies mechanical/electrical strategies promoting