Genetically Stable and Scalable Nanoengineering of Human Primary T Cells via Cell Mechanoporation

• DOI: 10.1021/acs.nanolett.3c01720
• Publication Date: 2023-07-28T17:17:47Z
• Authors: Jeongsoo Hur; Hyelee Kim; Uijin Kim; Gi-Beom Kim; Jinho Kim; Byeongju Joo; Duck Cho; Dong-Sung Lee; Aram J. Chung
• Keywords: Nanoengineering; Primary cell; Internalization

Effective tumor regression has been observed with chimeric antigen receptor (CAR) T cells; however, the development of an affordable, safe, and effective CAR-T cell treatment remains a challenge. One major obstacles is that suboptimal genetic modification cells reduces their yield antitumor activity, necessitating next-generation engineering approach. In this study, we developed nonviral nanoengineering system allows highly efficient delivery diverse functional nanomaterials into primary human in genetically stable scalable manner. Our platform leverages unique deformation restoration process induced by intrinsic inertial flow microchannel to create nanopores cellular membrane for macromolecule internalization, leading transfection high scalability viability. The proposed approach demonstrates considerable potential as practical alternative technique improving current manufacturing process.