Reversible Assembly of Proteins and Phenolic Polymers for Intracellular Protein Delivery with Serum Stability

• DOI: 10.1021/acs.nanolett.4c00937
• Publication Date: 2024-04-15T13:36:25Z
• Authors: Guangyu Rong; Xujiao Zhou; Jiaxu Hong; Yiyun Cheng

The design of intracellular delivery systems for protein drugs remains a challenge due to limited efficacy and serum stability. Herein, we propose reversible assembly strategy assemble cargo proteins phenolic polymers into stable nanoparticles this purpose using heterobifunctional adaptor (2-formylbenzeneboronic acid). is easily decorated on via iminoboronate chemistry further conjugates with catechol-bearing form boronate diester linkages. exhibit excellent stability in culture media but rapidly release the triggered by lysosomal acidity GSH after endocytosis. In proof-of-concept animal model, successfully transports superoxide dismutase retina intravitreal injection efficiently ameliorates oxidative stress cellular damage induced ischemia-reperfusion (I/R) minimal adverse effects. represents robust efficient method develop serum-stable biomacromolecules.