Simultaneous Electro-Optical Tracking for Nanoparticle Recognition and Counting

• DOI: 10.1021/acs.nanolett.5b01243
• Publication Date: 2015-07-30T21:24:07Z
• Authors: Elena Angeli; Andrea Volpe; Paola Fanzio; Luca Repetto; Giuseppe Firpo; Patrizia Guida; Roberto Lo Savio; Meni Wanunu; Ugo Valbusa
• Keywords: Electrophoresis; Optics and Photonics; Video Recording; Equipment Design; 01 natural sciences; 0104 chemical sciences; Diffusion; Lab-On-A-Chip Devices; Nanoparticles; Nanotechnology; Dimethylpolysiloxanes; nanoparticle diffusion; polymeric nanochannels; resistive pulse sensing; Single particle tracking; translocation dynamics; Condensed Matter Physics; Bioengineering; Chemistry (all); Materials Science (all); Mechanical Engineering; Particle Size; Fluorescent Dyes

We present the first detailed experimental observation and analysis of nanoparticle electrophoresis through a nanochannel obtained with synchronous high-bandwidth electrical and camera recordings. Optically determined particle diffusion coefficients agree with values extracted from fitting electrical transport measurements to distributions from 1D Fokker-Planck diffusion-drift theory. This combined tracking strategy enables optical recognition and electrical characterization of nanoparticles in solution, which can have a broad range of applications in biology and materials science.