High Density Individually Addressable Nanowire Arrays Record Intracellular Activity from Primary Rodent and Human Stem Cell Derived Neurons

• DOI: 10.1021/acs.nanolett.6b04752
• Publication Date: 2017-04-06T22:01:44Z
• Authors: Ren Liu; Renjie Chen; Ahmed T. Elthakeb; Sang Heon Lee; Sandy Hinckley; Massoud L. Khraiche; John Scott; Deborah Pre; Yoontae Hwang; Atsunori Tanaka; Yun Goo Ro; Albert K. Matsushita; Xing Dai; Cesare Soci; Steven Biesmans; Anthony James; John Nogan; Katherine L. Jung...; Douglas V. Pete; Denise B. Webb; Yimin Zou; Anne G. Bang; Shadi A. Dayeh
• Keywords: Neurite

We report a new hybrid integration scheme that offers for the first time nanowire-on-lead approach, which enables independent electrical addressability, is scalable, and has superior spatial resolution in vertical nanowire arrays. The fabrication of these arrays demonstrated to be scalable down submicrometer site-to-site spacing can combined with standard integrated circuit technologies. utilize perform electrophysiological recordings from mouse rat primary neurons human induced pluripotent stem cell (hiPSC)-derived neurons, revealed high signal-to-noise ratios sensitivity subthreshold postsynaptic potentials (PSPs). measured activity rodent 8 days vitro (DIV) 14 DIV hiPSC-derived at 6 weeks post culture signal amplitudes up 99 mV. Overall, our platform paves way longitudinal experiments on synaptic iPSC based disease models neuronal networks, critical understanding mechanisms neurological diseases developing drugs treat them.