Intracellular Delivery of Nanomaterials via an Inertial Microfluidic Cell Hydroporator

• DOI: 10.1021/acs.nanolett.8b00704
• Publication Date: 2018-03-23T13:35:44Z
• Authors: Yanxiang Deng; Megan Kizer; Miran Rada; Jessica Sage; Xing Wang; Dong-Joo Cheon; Aram J. Chung
• Keywords: Nanomaterials; Nanocarriers

The introduction of nanomaterials into cells is an indispensable process for studies ranging from basic biology to clinical applications. To deliver foreign living cells, traditionally endocytosis, viral and lipid nanocarriers or electroporation are mainly employed; however, they critically suffer toxicity, inconsistent delivery, low throughput time-consuming labor-intensive processes. Here, we present a novel inertial microfluidic cell hydroporator capable delivering wide range various types in single-step without the aid carriers external apparatus. platform inertially focuses channel center guides collide at T-junction. Controlled compression shear forces generate transient membrane discontinuities that facilitate passive diffusion cytoplasm while maintaining high viability. This hydroporation method shows superior delivery efficiency, high-throughput, has controllability; moreover, its extremely simple low-cost operation provides powerful practical strategy applications cellular imaging, biomanufacturing, cell-based therapies, regenerative medicine, disease diagnosis.