Engineering of Dual-Function Vitreoscilla Hemoglobin: A One-Pot Strategy for the Synthesis of Unnatural α-Amino Acids

• DOI: 10.1021/acs.orglett.3c02537
• Publication Date: 2023-09-22T12:08:43Z
• Authors: Fengxi Li; Yaning Xu; Yuelin Xu; Hanqing Xie; Junhao Wu; Chunyu Wang; Zhengqiang Li; Zhi Wang; Lei Wang
• Keywords: Hemeprotein; Dual function

Despite a well-developed and growing body of work on the directed evolution hemoproteins, potential hemoproteins to catalyze non-natural reactions remains underexplored. This paper reports new biocatalytic strategy for one-pot synthesis unnatural α-amino acids. Engineered variants dual-function Vitreoscilla hemoglobin were found efficiently N-H insertion C-H sp3 alkylation, providing moderate excellent yields (57%-95%) acid derivatives turnover numbers (1425-2375).