Identification and Characterization of Enzymes Catalyzing Pyrazolopyrimidine Formation in the Biosynthesis of Formycin A

• DOI: 10.1021/acs.orglett.7b00355
• Publication Date: 2017-02-24T12:26:09Z
• Authors: Yeonjin Ko; Shao-An Wang; Yasushi Ogasawara; Mark W. Ruszczycky; Hung-wen Liu
• Keywords: Pyrimidine metabolism

Genome scanning of Streptomyces kaniharaensis, the producer formycin A, reveals two sets purA, purB, purC, and purH genes. The Pur enzymes catalyze pyrimidine assembly purine nucleobases. To test whether encoded by second set pur genes analogous transformations in biosynthesis, B 5′-phosphate was synthesized shown to be converted ForA ForB A 5′-phosphate. These results support that For are responsible for formation.