Zinc Silicate/Nano-Hydroxyapatite/Collagen Scaffolds Promote Angiogenesis and Bone Regeneration via the p38 MAPK Pathway in Activated Monocytes

• DOI: 10.1021/acsami.0c00470
• Publication Date: 2020-03-17T21:03:17Z
• Authors: Yue Song; Hao Wu; Yi Gao; Junqin Li; Kaifeng Lin; Bin Liu; Xing Lei; Pengzhen Cheng; Shuaishuai Zhang; Yixiao Wang; Jinbo Sun; Long Bi; Guoxian Pei
• Keywords: Homing (biology)

Recent studies show that biomaterials are capable of regulating immune responses to induce a favorable osteogenic microenvironment and promote osteogenesis angiogenesis. In this study, we investigated the effects zinc silicate/nanohydroxyapatite/collagen (ZS/HA/Col) scaffolds on bone regeneration angiogenesis explored related mechanism. We demonstrate 10ZS/HA/Col significantly enhanced in vivo compared with HA/Col scaffolds. ZS/HA/Col increased tartrate-resistant acid phosphatase (TRAP)-positive cells, nestin-positive marrow stromal cells (BMSCs) CD31-positive neovessels, expression (Bmp-2 Osterix) angiogenesis-related (Vegf-α Cd31) genes nascent bone. 10 wt % ZS activated p38 signaling pathway monocytes. The monocytes subsequently differentiated into TRAP+ expressed higher levels cytokines SDF-1, TGF-β1, VEGF-α, PDGF-BB, which recruited BMSCs endothelial (ECs) defect areas. Blocking reduced differentiation cytokine secretion resulted decrease BMSC EC homing Overall, these findings modulate and, thereby, create promotes migration vessel formation by activating pathway.