Complementary Oligonucleotide Conjugated Multicolor Carbon Dots for Intracellular Recognition of Biological Events

• DOI: 10.1021/acsami.0c02463
• Publication Date: 2020-03-17T22:25:06Z
• Authors: Indrajit Srivastava; Santosh K. Misra; Sushant Bangru; Kingsley A. Boateng; Julio A. N. T. So...; Aaron S. Schwartz...; Auinash Kalsotra; Dipanjan Pan
• Keywords: Carbon fibers

By using complementary DNA sequences as surface ligands, we selectively allow two individual diffusing "dual-color" carbon dots to interact in situ and vitro. Spontaneous nanoscale oxidation of surface-abundant nitroso-/nitro-functionalities leads distinctly colored (CD) which are isolated by polarity driven chromatographic separation. Green- red-emitting (gCD rCD) were decorated single-stranded DNAs produce a marked increase the fluorescence emission respective dots. Mutual colloidal interactions achieved through hybridization base pairs attached particles, resulting quenching their photoluminescence. The observed post-hybridization is presumably due combined effect from an aggregation CDs post duplex formation close proximity multicolored CDs, having overlapped spectral regions leading nonradiative energy transfer process possibly released heat. This strategy may contribute rational design mutually interacting for better control over assembly structure studying different biological phenomenon including molecular cytogenetics. One newly synthesized was successfully used image intracellular location GAPDH mRNA event change intensity (FI) CDs. selectivity introduced conjugating oligonucleotide harboring sequence mRNA. FI this conjugated dot, rCD-GAPDH, also found decrease presence Ca2+, varied relation H+ concentrations, could serve tool quantify concentrations Ca2+ pH value (H+) can give important information about cell survival. Therefore, CD-oligonucleotide conjugates efficient probes cellular events interventions.