Preparation of Gas-Responsive Imprinting Hydrogel and Their Gas-Driven Switchable Affinity for Target Protein Recognition

• DOI: 10.1021/acsami.0c05561
• Publication Date: 2020-05-05T06:06:45Z
• Authors: Yubo Wei; Qiang Zeng; Jianzhi Huang; Xinrong Guo; Lulu Wang; Lishi Wang
• Keywords: Molecular imprinting; Human serum albumin; Molecular Recognition

Novel gas-responsive imprinting hydrogels were fabricated by combining N,N′-dimethylaminoethyl methacrylate gas-sensitive monomers, N,N′-methylenebis(acrylamide) cross-linkers, and human serum albumin (HSA) template proteins via a free radical polymerization. The hydrogel exhibited reversible property upon N2/CO2 exchange. This result was supported the evidences from hydrogen nuclear magnetic resonance spectroscopy scanning electron microscopy. By applying this to sensing application, CO2-responsive imprinted biosensor originally designed on surface of glassy carbon electrode. unique self-clean self-recognition properties toward HSA based conformational changes driven stimuli. Moreover, proposed favored showing satisfactory sensitivity selectivity wider detection range with low limit. As rare example in imprint sensing, successfully applied extraction complex samples. With gas stimuli, whole process efficient, controllable, harmless proteins. Thus, developed may provide new prospect molecularly applications.