Designer Hydrogel with Intelligently Switchable Stem-Cell Contact for Incubating Cartilaginous Microtissues

• DOI: 10.1021/acsami.0c13426
• Publication Date: 2020-08-17T07:04:23Z
• Authors: Zijie Xiahou; Yunlang She; Jiahui Zhang; Yechi Qin; Guifei Li; Lili Zhang; Haowei Fang; Kunxi Zhang; Chang Chen; Jingbo Yin
• Keywords: Polylactic Acid; Chondrogenesis

Stem-cell-derived organoid can resemble in vivo tissue counterpart and mimic at least one function of or organ, possessing great potential for biomedical application. The present study develops a hydrogel with cell-responsive switch to guide spontaneous sequential proliferation aggregation adipose-derived stem cells (ASCs) without inputting artificial stimulus vitro constructing cartilaginous microtissues enhanced retention cell–matrix cell–cell interactions. Polylactic acid (PLA) rods are surface-aminolyzed by cystamine, followed being involved the amidation poly(( l-glutamic acid) adipic dihydrazide (ADH) form hydrogel. Along tubular pore formation after dissolution PLA rods, aminolyzed molecules disulfide bonds on rod surfaces covalently transferred poly(l-glutamic acid)/ADH Because attaches cells, while repels ASCs found adhere proliferate first then cleave secreting containing thiol, thus inducing desorption leading their detachment aggregation. Associated chondrogenic induction TGF-β1 IGF-1 28 days, as an all-in-one incubator produces well-engineered columnar cartilage from ASCs, glycosaminoglycans (GAGs) collagen type II (COL II) deposition achieving 64 69% those chondrocytes pellet, respectively. further matured 8 weeks exhibit extremely similar histological features biomechanical performance native hyaline cartilage. GAGs COL content, well compressive modulus show no significant difference designer may hold promise long-term culture other types organoids.