Delicately Designed Cancer Cell Membrane-Camouflaged Nanoparticles for Targeted 19F MR/PA/FL Imaging-Guided Photothermal Therapy

• DOI: 10.1021/acsami.0c13865
• Publication Date: 2020-11-25T04:13:56Z
• Authors: Sha Li; Weiping Jiang; Yaping Yuan; Meiju Sui; Yuqi Yang; Liqun Huang; Ling Jiang; Maili Liu; Shizhen Chen; Xin Zhou
• Keywords: Indocyanine Green; Male; Fluorocarbons; Mice, Inbred BALB C; Infrared Rays; Photothermal Therapy; Cell Membrane; Optical Imaging; Antineoplastic Agents; Fluorine; 02 engineering and technology; Magnetic Resonance Imaging; Xenograft Model Antitumor Assays; Theranostic Nanomedicine; 3. Good health; Photoacoustic Techniques; Polylactic Acid-Polyglycolic Acid Copolymer; A549 Cells; Neoplasms; Animals; Humans; Nanoparticles; 0210 nano-technology

Our exploration of multimodal nanoprobes aims to combine photoacoustic (PA) imaging, 19F magnetic resonance (MR), and fluorescence (FL) imaging, which offers complementary advantages such as high spatial resolution, unlimited penetration, and high sensitivity to enable more refined images for accurate tumor diagnoses. In this research, perfluorocarbons (PFCs) and indocyanine green (ICG) are encapsulated by poly(lactic-co-glycolic acid) (PLGA) for intravital 19F MR/FL/PA tri-modal imaging-guided photothermal therapy. Then, it is coated with an A549 cancer cell membrane (AM) to fabricate versatile theranostic nanoprobes (AM-PP@ICGNPs). After systemic administration, FLI reveals time-dependent tumor homing of NPs with high sensitivity, 19F MRI provides tumor localization of NPs without background signal interference, and PAI illustrates the detailed distribution of NPs inside the tumor with high spatial resolution. What is more, AM-PP@ICGNPs accumulated in the tumor area exhibit a prominent photothermal effect (48.4 °C) under near infrared (NIR) laser irradiation and realize an enhanced antitumor response in vivo. These benefits, in combination with the excellent biocompatibility, make AM-PP@ICGNPs a potential theranostic nanoagent for accurate tumor localization and ultimately achieve superior cancer therapy.