Vortex Fluidic-Mediated Fabrication of Fast Gelated Silica Hydrogels with Embedded Laccase Nanoflowers for Real-Time Biosensing under Flow

• DOI: 10.1021/acsami.0c15669
• Publication Date: 2020-11-05T19:55:55Z
• Authors: Xuan Luo; Ahmed Hussein Moh...; Aghil Igder; Keith A. Stubbs; Peng Su; Wei Zhang; Gregory A. Weiss; Colin L. Raston
• Keywords: Fluidics

The fabrication of hybrid protein-Cu3(PO4)2 nanoflowers (NFs) via an intermediate toroidal structure is dramatically accelerated under shear using a vortex fluidic device (VFD), which possesses rapidly rotating angled tube. As-prepared laccase NFs (LNFs) exhibit ≈1.8-fold increase in catalytic activity compared to free diffusion control, further enhanced by ≈ 2.9-fold for the catalysis VFD. A new LNF immobilization platform, LNF@silica incorporated VFD tube, was subsequently developed mixing LNFs 15 min with silica hydrogel resulting gelation along tube surface. LNFs@silica coating highly stable and reusable, allows dramatic 16-fold enhancement rates relative inside glass vials. Ultraviolet–visible spectroscopy-based real-time monitoring within LNFs@silica-coated reveals good stability continuous flow processing. results demonstrate utility microfluidic highlighting its ability control chemical enzymatic processes.