Organic Solvent-Free Preparation of Chitosan Nanofibers with High Specific Surface Charge and Their Application in Biomaterials

CSNF Chemical Sciences not elsewhere classified Immunology chitosan nanofibers Static Electricity Biophysics Nanofibers Plant Biology Biocompatible Materials 02 engineering and technology Biochemistry Cell Line Mice Cell Adhesion Animals Molecular Biology Pharmacology Chitosan Osteoblasts Ecology Tissue Engineering Tissue Scaffolds rootlike chitosan nanofibers chitosan nanofiber paper Organic Solvent-Free Preparation Hematology chitosan films method chitosan nanofiber scaffold products CSNFP 0210 nano-technology Physical Sciences not elsewhere classified calvaria-derived preosteoblastic cells Biotechnology Developmental Biology
DOI: 10.1021/acsami.0c21796 Publication Date: 2021-02-24T16:23:00Z
ABSTRACT
The application of chitosan nanofibers in biological tissue-engineering materials has attracted wide attention. A novel and organic solvent-free method was developed for the fabrication of rootlike chitosan nanofibers (CSNFs) with diameters of 40-250 nm. This method includes three-step mechanical processing of swelling-beating-centrifugation or swelling-beating-homogenization. The obtained nanofibers showed high yields (>95%) and positive specific surface charges (up to +375 μeq/g) and could be uniformly dispersed in the aqueous phase. The unique fiber shape and the good length-to-diameter ratio of CSNFs endowed chitosan nanofiber paper (CSNFP) products with excellent mechanical properties, and the wet tensile strength of the CSNFPs was nearly five times higher than common chitosan films. In addition, the calvaria-derived preosteoblastic cells exhibited a higher adherence efficiency and proliferation on CSNFP than on chitosan films. The chitosan nanofiber scaffold products also benefited the attachment of preosteoblastic cells and allowed them to grow in three dimensions. This method has significant industrial potential for the industrialization of chitosan nanofibers, which may have broad applications in various biomaterials.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (67)
CITATIONS (16)