Organic Solvent-Free Preparation of Chitosan Nanofibers with High Specific Surface Charge and Their Application in Biomaterials
CSNF
Chemical Sciences not elsewhere classified
Immunology
chitosan nanofibers
Static Electricity
Biophysics
Nanofibers
Plant Biology
Biocompatible Materials
02 engineering and technology
Biochemistry
Cell Line
Mice
Cell Adhesion
Animals
Molecular Biology
Pharmacology
Chitosan
Osteoblasts
Ecology
Tissue Engineering
Tissue Scaffolds
rootlike chitosan nanofibers
chitosan nanofiber paper
Organic Solvent-Free Preparation
Hematology
chitosan films
method
chitosan nanofiber scaffold products
CSNFP
0210 nano-technology
Physical Sciences not elsewhere classified
calvaria-derived preosteoblastic cells
Biotechnology
Developmental Biology
DOI:
10.1021/acsami.0c21796
Publication Date:
2021-02-24T16:23:00Z
AUTHORS (8)
ABSTRACT
The application of chitosan nanofibers in biological tissue-engineering materials has attracted wide attention. A novel and organic solvent-free method was developed for the fabrication of rootlike chitosan nanofibers (CSNFs) with diameters of 40-250 nm. This method includes three-step mechanical processing of swelling-beating-centrifugation or swelling-beating-homogenization. The obtained nanofibers showed high yields (>95%) and positive specific surface charges (up to +375 μeq/g) and could be uniformly dispersed in the aqueous phase. The unique fiber shape and the good length-to-diameter ratio of CSNFs endowed chitosan nanofiber paper (CSNFP) products with excellent mechanical properties, and the wet tensile strength of the CSNFPs was nearly five times higher than common chitosan films. In addition, the calvaria-derived preosteoblastic cells exhibited a higher adherence efficiency and proliferation on CSNFP than on chitosan films. The chitosan nanofiber scaffold products also benefited the attachment of preosteoblastic cells and allowed them to grow in three dimensions. This method has significant industrial potential for the industrialization of chitosan nanofibers, which may have broad applications in various biomaterials.
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