3D-Bioprinted Difunctional Scaffold for In Situ Cartilage Regeneration Based on Aptamer-Directed Cell Recruitment and Growth Factor-Enhanced Cell Chondrogenesis

• DOI: 10.1021/acsami.1c01844
• Publication Date: 2021-05-12T20:13:14Z
• Authors: Zhen Yang; Tianyuan Zhao; Cangjian Gao; Fuyang Cao; Hao Li; Zhiyao Liao; Liwei Fu; Pinxue Li; Wei Chen; Zhiqiang Sun; Shuangpeng Jiang; Zhuang Tian; Guangzhao Tian; Kangkang Zha; Tingting Pan; Xu Li; Xiang Sui; Zhiguo Yuan; Shuyun Liu; Quanyi Guo
• Keywords: Chondrogenesis; 3D bioprinting; Decellularization; Hyaline cartilage; Aptamer

Articular cartilage (AC) lesions are fairly common but remain an obstacle for clinicians and researchers due to their poor self-healing capacity. Recently, a promising therapy based on the recruitment of autologous mesenchymal stem cells (MSCs) has been developed regeneration full-thickness defects in knee joint. In this study, 3D-bioprinted difunctional scaffold was aptamer HM69-mediated MSC-specific growth factor-enhanced cell chondrogenesis. The aptamer, which can specifically recognize recruit MSCs, first chemically conjugated decellularized extracellular matrix then mixed with gelatin methacrylate form photocrosslinkable bioink ready 3D bioprinting. Together factor that promoted chondrogenic differentiation, biodegradable polymer poly(ε-caprolactone) further chosen impart mechanical strength bioprinted constructs. recruited provided favorable microenvironment adhesion proliferation, chondrogenesis, thus greatly improved repair rabbit defects. conclusion, study demonstrated bioprinting scaffolds could be strategy situ AC aptamer-directed growth-factor-enhanced