Rational Design of Dendritic Mesoporous Silica Nanoparticles’ Surface Chemistry for Quantum Dot Enrichment and an Ultrasensitive Lateral Flow Immunoassay

• DOI: 10.1021/acsami.1c02149
• Publication Date: 2021-05-03T16:37:54Z
• Authors: Fang Gao; Chang Lei; Yang Liu; Hao Song; Yueqi Kong; Jingjing Wan; Chengzhong Yu
• Keywords: Thiol; Passivation; Naked eye; Surface Modification

Lateral flow immunoassays (LFIAs) have drawn much attention in point-of-care diagnostic applications, and the development of high-performance label materials is key. In this study, impact surface chemistry dendritic mesoporous silica nanoparticles (DMSNs) on their enrichment performance toward quantum dots (QDs) signal amplification resultant DMSNs-QDs as been investigated. A series DMSNs with controllable amino/thiol group densities synthesized. It demonstrated that amino groups are beneficial for QD fluorescence preservation, owing to amino-based passivation, while thiol responsible increasing loading capacity QDs due thiol–metal coordination. The optimized labels an density 153 μmol g–1 a 218 displayed sufficient preservation (89.4%) high (1.55 g g–1). Ultrasensitive detection serum amyloid (SAA) limit 10 pg mL–1 naked eye was achieved, which 1 order magnitude higher than reported literature. This study provides insights into advanced ultrasensitive LFIA future bioassay applications.