Development of Cellulose Nanofibril/Casein-Based 3D Composite Hemostasis Scaffold for Potential Wound-Healing Application

• DOI: 10.1021/acsami.1c21039
• Publication Date: 2022-01-17T10:49:52Z
• Authors: Santosh Shivaji B...; Jianzhong Sun; Lu Cheng; Yu Cheng; Yifei Shi; Sujie Yu; Haixin Jiao; Meng Zhang; Xuechu Lu; Wenjia Han; Qianqian Wang; Zhen Zhang; Jun Liu
• Keywords: Wound Healing; Tissue Scaffolds; Materials Testing; Printing, Three-Dimensional; Nanofibers; Caseins; Humans; Biocompatible Materials; 02 engineering and technology; Cellulose; 0210 nano-technology

Excessive bleeding in traumatic hemorrhage is the primary concern for natural wound healing and main reason trauma deaths. The three-dimensional (3D) bioprinting of bioinks offers desired structural complexity vital hemostasis activity targeted cell proliferation rapid controlled healing. However, it challenging to develop suitable fabricate specific 3D scaffolds desirable In this work, a composite scaffold designed using technology synergistic mechanisms cellulose nanofibrils (TCNFs), chitosan, casein control blood loss hemorrhage. Bioinks that consist bioconjugated TCNF (with content 104.5 ± 34.1 mg/g) carbodiimide cross-linker chemistry were subjected customizable fabrication. Further, situ cross-linked green ionic complexation approach. covalent conjugation among TCNF, casein, chitosan was confirmed by Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), X-ray photoelectron spectroscopy (XPS), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), diffraction (XRD) studies. vitro analyzed human thrombin-antithrombin (TAT) assay adsorption red cells (RBCs) platelets. had better swelling behavior faster whole clotting rate at each time point than commercial cellulose-based dressings. TAT demonstrated could form higher thrombin (663.29 pg/mL) stable clot compared cellulosic pad (580.35 pg/mL), (457.78 gauze (328.92 which are essential coagulation. addition, lower index (23.34%) (41.93%), suggesting efficiencies RBC entrapping induce clotting. vivo cytocompatibility evaluated culture study, results showed promote growth NIH 3T3 fibroblast cells, Cellulase-based deconstruction significant weight (80 5%) lysozyme hydrolysis (22 after 28 days incubation, biodegradation potential scaffold. conclusion, study proposes efficient prospects from TCNF-based can accelerate healing, its application reducing during