Simultaneous Imaging of Dual microRNAs in Cancer Cells through Catalytic Hairpin Assembly on a DNA Tetrahedron

• DOI: 10.1021/acsami.1c23227
• Publication Date: 2022-02-25T20:03:00Z
• Authors: Chun Hong Li; Wen Yi Lv; Fei Fan Yang; Shu Jun Zhen; Cheng Zhi Huang
• Keywords: Aptamer; Nucleolin

Accurate detection and imaging of tumor-related microRNA (miRNA) in living cells hold great promise for early cancer diagnosis prognosis. One the challenges is to develop methods that enable identification multiple miRNAs simultaneously further improve accuracy. Herein, a simultaneous method two was established by using programmable designed DNA tetrahedron nanostructure (DTN) probe includes nucleolin aptamer (AS1411), miRNA capture strands, pairs metastable catalytic hairpins at different vertexes. The DTN exhibited enhanced tumor cell recognition ability, excellent stability biocompatibility, fast reaction kinetics. It found could specifically enter cells, which strand hybridize with initiate hairpin assembly (CHA) only when overexpressed miR-21 miR-155 existed simultaneously, resulting distinct fluorescence resonance energy transfer signal demonstrating feasibility this diagnosis.