Efficient Tumor Immunotherapy through a Single Injection of Injectable Antigen/Adjuvant-Loaded Macroporous Silk Fibroin Microspheres

• DOI: 10.1021/acsami.2c11286
• Publication Date: 2022-09-16T15:49:45Z
• Authors: Fang Lei; Jiangfeng Cai; Ruyin Lyu; Honglan Shen; Yalan Xu; Jie Wang; Yajun Shuai; Zongpu Xu; Chuanbin Mao; Mingying Yang
• Keywords: 0301 basic medicine; 03 medical and health sciences; Adjuvants, Immunologic; Silk; Animals; Immunotherapy; 02 engineering and technology; Bombyx; Fibroins; 0210 nano-technology; Microspheres; Adjuvants, Pharmaceutic; 3. Good health

Synthetic or natural materials have been used as vaccines in cancer immunotherapy. However, using them as vaccines necessitates multiple injections or surgical implantations. To tackle such daunting challenges, we develop an injectable macroporous Bombyx mori (B. mori) silk fibroin (SF) microsphere loaded with antigens and immune adjuvants to suppress established tumors with only a single injection. SF microspheres can serve as a scaffold by injection and avoid surgical injury as seen in traditional scaffold vaccines. The macroporous structure of the vaccine facilitates the recruitment of immune cells and promotes the activation of dendritic cells (DCs), resulting in a favorable immune microenvironment that further induces strong humoral and cellular immunity. We have also modified the vaccine into a booster version by simply allowing the antigens to be adsorbed onto the SF microspheres. The booster vaccine highly efficiently suppresses tumor growth by improving the cytotoxic T lymphocyte (CTL) response. In general, these results demonstrate that the macroporous SF microspheres can serve as a facile platform for tumor vaccine therapy in the future. Since the SF microspheres are also potential scaffolds for tissue regeneration, their use as a vaccine platform will enable their applications in eradicating tumors while regenerating healthy tissue to heal the tumor-site cavity.