Biomimetic Extracellular Matrix Nanofibers Electrospun with Calreticulin Promote Synergistic Activity for Tissue Regeneration

• DOI: 10.1021/acsami.2c13887
• Publication Date: 2022-11-10T19:58:48Z
• Authors: Mary E. Stack; Sarita Mishra; Matangi Parimala ...; Haoyu Wang; Leslie I. Gold; Hongjun Wang
• Keywords: Electrospinning; Vinculin; Polycaprolactone

In recognition of the potential calreticulin (CRT) protein in enhancing rate and quality wound healing excisional animal models, this study was to incorporate CRT via polyblend electrospinning into polycaprolactone (PCL)/type 1 collagen (Col1) nanofibers (NFs; 334 ± 75 nm diameter) as biomimetic extracellular matrices provide a novel mode delivery protection with enhanced synergistic activities for tissue regeneration. Release kinetic studies using fluoresceinated (CRT-FITC) NFs showed burst release within 4 h reaching plateau at 72 h, further intervals upon incubation fresh phosphate buffered saline up 8 weeks. By measuring fluorescence during first release, CRT-FITC-containing were shown protect from proteolytic digestion (e.g., by subtilisin) compared CRT-FITC solution. incorporated (CRT-NFs) also retention biological potency stimulating proliferation migration human keratinocytes fibroblasts. Fibroblasts seeded on CRT-NFs, after 2 days, increased amounts fibronectin, TGF-β1, integrin β1 cell lysates immunoblotting. Compared without CRT, CRT-NFs supported responses consistent greater polarization laminin-5 deposition more motile phenotype fibroblasts, suggested vinculin-capping F-actin fibers nonuniformly located throughout body secretion phosphorylated focal adhesion kinase-enriched migrasomes. Altogether, electrospun PCL/Col1 retained its structural integrity functions while having additional benefits customizable loading, degradation, sustained NFs, coupled innate physicochemical cues NFs. Such have recalcitrant wounds such diabetic foot ulcers.