Assembled Photonic Crystal/Gold Nanoparticle Interface: A Dual Amplifying Electrochemiluminescent Aptasensor for the Ultrasensitive Detection of an Amyloid-β Monomer

• DOI: 10.1021/acsami.2c17342
• Publication Date: 2023-02-10T03:56:33Z
• Authors: Rong Tan; Yuhong Liu; Yueju Wang; Huiling Li; Yifeng Tu
• Keywords: Luminol; Electrochemiluminescence; Aptamer; SIGNAL (programming language)

Amyloid-β (Aβ) protein is considered to be a key biomarker that closely associated with Alzheimer's disease (AD). The level of Aβ, particularly its subtle fluctuation, indicates early neuropathological changes, which poses considerable challenge in predicting AD, considering the detection limit sensing technologies. Herein, new label-free sensor based on luminol electrochemiluminescence (ECL) was proposed by developing close-packed monolayered-SiO2 array gold (Au) nanoparticles (NPs) entrapped their gaps as basal electrode. well-organized SiO2 NPs quasiphotonic crystal structure amplified ECL signal via light scattering, while Au directly catalyzing oxidation. Owing dual amplification, electrode furnished an ∼64-fold-intensified background. Further, as-prepared served substrate develop aptasensor for sensitive Aβ. inhibition due suppressed diffusion surface acts indicator quantify amount transfer dynamics mechanism provides strategy and facilitates high sensitivity Aβ detection. Under optimal conditions, developed exhibits ultrasensitive performance very low 5 fM, providing prospect clinical research promising approach field sensing.