Lossless Immunocytochemistry Based on Large-Scale Porous Hydrogel Pellicle for Accurate Rare Cell Analysis

• DOI: 10.1021/acsami.2c18321
• Publication Date: 2023-02-22T04:44:29Z
• Authors: Hyeong Jung Woo; Seung-Hoon Kim; Hyun Gyu Kang; Taehoon Kim; Sooyeol Kim; Jong Man Kim; Jae Young Kim; Seung Joon Lee; Young Zoon Kim; So Yeon Oh; Ji Hyae Lim; Hyun Mee Ryu; Minseok S. Kim
• Keywords: 0301 basic medicine; rare cell analysis; BLOOD; 610; Hydrogels; intact cell morphology; circulating tumor cell; Immunohistochemistry; CIRCULATING TUMOR-CELLS; 3. Good health; 620; 03 medical and health sciences; immunocytochemistry; lossless cell staining; circulating fetal cell; Neoplasms; single-cell analysis; Humans; hydrogel; Porosity

Rare cells, such as circulating tumor cells or fetal provide important information for the diagnosis and prognosis of cancer prenatal diagnosis. Since undercounting only a few can lead to significant misdiagnosis incorrect decisions in subsequent treatment, it is crucial minimize cell loss, particularly rare cells. Moreover, morphological genetic on should be preserved intact possible downstream analysis. The conventional immunocytochemistry (ICC), however, fails meet these requirements, causing unexpected loss deformation organelles which may mislead classification benign malignant In this study, novel ICC technique preparing lossless cellular specimens was developed improve diagnostic accuracy analysis analyze morphology. To end, robust reproducible porous hydrogel pellicle developed. This encapsulates from repeated exchange reagents prevent deformation. soft allows stable picking further analysis, difficult with methods that permanently immobilize platform will pave way precise toward clinical practice.