Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes

• DOI: 10.1021/acsami.2c22551
• Publication Date: 2023-03-30T06:05:21Z
• Authors: Feng Tian; Linlin Yin; Peiran Lin; Yurong Liu; Wenlong Wang; Yong Chen; Yadong Tang
• Keywords: [SPI]Engineering Sciences [physics]; 330; [SDV]Life Sciences [q-bio]; Induced Pluripotent Stem Cells; Nanofibers; [CHIM]Chemical Sciences; Humans; Endothelial Cells; Myocytes, Cardiac; Cell Differentiation; Endothelium; Cells, Cultured

Cell alignment widely exists in various vivo tissues and also plays an essential role the construction of vitro models, such as vascular endothelial myocardial models. Recently, microscale nanoscale hierarchical topographical structures have been drawing increasing attention for engineering cell alignment. In present study, we fabricated a micro-/nanohierarchical substrate based on soft lithography electrospinning to assess synergetic effect both aligned nanofibrous guidance off-ground culture environment provided by endothelium formation maturation human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The morphology, proliferation, barrier human umbilical vein cells (HUVECs) well alignment, cardiac-specific proteins, maturity-related gene expression hiPSC-CMs aligned-nanofiber/microridge (AN-MR) were studied. Compared with glass slide single-aligned nanofiber substrate, AN-MR enhanced cell-cell interaction HUVECs improved length sarcomere maturation-related hiPSC-CMs. Finally, response different substrates two typical cardiac drugs (isoproterenol E-4031) was tested analyzed, showing that more resistant than those other groups, which related higher maturity cells. Overall, proposed supports enhances hiPSC-CMs, show great potential be applied models tissue engineering.