Self-assembled GA-Repeated Peptides as a Biomolecular Scaffold for Biosensing with MoS2 Electrochemical Transistors

• DOI: 10.1021/acsami.2c23227
• Publication Date: 2023-03-09T13:48:41Z
• Authors: Hironaga Noguchi; Yoshiki Nakamura; Sayaka Tezuka; Takakazu Seki; Kazuki Yatsu; Takuma Narimatsu; Yasuaki Nakata; Yuhei Hayamizu
• Keywords: Transconductance; Alanine

Biosensors with two-dimensional materials have gained wide interest due to their high sensitivity. Among them, single-layer MoS2 has become a new class of biosensing platform owing its semiconducting property. Immobilization bioprobes directly onto the surface chemical bonding or random physisorption been widely studied. However, these approaches potentially cause reduction conductivity and sensitivity biosensor. In this work, we designed peptides that spontaneously align into monomolecular-thick nanostructures on electrochemical transistors in non-covalent fashion act as biomolecular scaffold for efficient biosensing. These consist repeated domains glycine alanine sequence form self-assembled structures sixfold symmetry templated by lattice MoS2. We investigated electronic interactions designing amino acid charged acids at both ends. Charged showed correlation electrical properties MoS2, where negatively caused shift threshold voltage neutral positively had no significant effect voltage. The transconductance decrease peptides, indicating aligned can without degrading intrinsic also impact photoluminescence (PL) found PL intensity changed sensitively depending peptides. Finally, demonstrated femtomolar-level using biotinylated detect streptavidin.