Assembly of Reusable DNA Blocks for Data Storage Using the Principle of Movable Type Printing

• DOI: 10.1021/acsami.3c01860
• Publication Date: 2023-05-15T15:37:16Z
• Authors: Chengtao Xu; Biao Ma; Xing Dong; Lanjie Lei; Qing Hao; Chao Zhao; Hong Liu
• Keywords: DNA Computing; Primer (cosmetics)

Due to its high coding density and longevity, DNA is a compelling data storage alternative. However, current systems rely on the de novo synthesis of enormous molecules, resulting in low editability, costs, restrictions further applications. Here, we demonstrate programmable assembly reusable blocks for versatile using ancient movable type printing principle. Digital are first encoded into nucleotide sequences hairpins, which then synthesized immobilized solid beads as modular blocks. Using polymerase-catalyzed primer exchange reaction, can be continuously replicated from hairpins attached tandem produce new information. The highly programmable, producing various by reusing finite number reducing costs (∼1718 versus 3000 30,000 US$ per megabyte conventional methods). We flexible texts, images, random numbers integration with logic circuits manipulate synthesis. This work suggests paradigm recombining already build cost-effective intelligent systems.