Layer-by-Layer Deposition of Low-Solid Nanochitin Emulgels Creates Porous Structures for High Cell Attachment and Proliferation

• DOI: 10.1021/acsami.3c03421
• Publication Date: 2023-05-26T15:07:05Z
• Authors: Ya Zhu; Esko Kankuri; Xue Zhang; Zhangmin Wan; Xin Wang; Siqi Huan; Long Bai; Shouxin Liu; Orlando J. Rojas
• Keywords: Area density

Direct ink writing (DIW) is a customizable platform to engineer complex constructs from biobased colloids. However, the latter usually display strong interactions with water and lack interparticle connectivity, limiting one-step processing into hierarchically porous structures. We overcome such challenges by using low-solid emulgel inks stabilized chitin nanofibrils (nanochitin, NCh). By complementary characterization platforms, we reveal NCh structuring spatially controlled three-dimensional (3D) materials that generate multiscale porosities defined emulsion droplet size, ice templating, DIW infill density. The extrusion variables, key in development of surface mechanical features printed architectures, are comprehensively analyzed molecular dynamics other simulation approaches. obtained scaffolds shown for their hierarchical structures, high areal density, stiffness, which lead excellent modulation cell adhesion, proliferation, differentiation, as tested mouse dermal fibroblast expressing green fluorescent proteins.