Absolute Quantification of Serum Exosomes in Patients with an SERS-Lateral Flow Strip Biosensor for Noninvasive Clinical Cancer Diagnosis

• DOI: 10.1021/acsami.3c05039
• Publication Date: 2023-08-01T04:06:10Z
• Authors: Xiaoming Su; Yangcenzi Xie; Xinyu Liu; Mingyang Chen; Chao Zheng; Hong Zhong; Ming Li
• Keywords: Cancer Biomarkers

Exosomes (exos) widely existing in body fluids show great potential for noninvasive cancer diagnosis. Quantitative analysis of exos is traditionally performed by targeting specific exosomal surface proteins, but it often imprecise due to the common expression proteins and subtle differences between different subtypes. Herein, we report quantitative surface-enhanced Raman spectroscopy (SERS) serum through a combination paper-based lateral flow strip (LFS) biosensor with multivariate spectral unmixing rather than simply quantifying proteins. Our SERS-LFS enables absolute quantification two limit detection down ∼106 particles/mL both exos. We further exemplify application this strategy dual-plex from breast patients. find that human epidermal growth factor receptor 2+ (HER2+) luminal A patients undergoing no surgery are enriched derived SKBR-3 cells MCF-7 (denoted as SKBR MCF exos), respectively. The surgical treatment these accompanies an obvious decrease either or serum. These results suggest subtyping therapeutic surveillance powerful capability clinical samples.