In Vitro Study of the Anti-inflammatory and Antifibrotic Activity of Tannic Acid-Coated Curcumin-Loaded Nanoparticles in Human Tenocytes

• DOI: 10.1021/acsami.3c05322
• Publication Date: 2023-05-02T15:32:46Z
• Authors: Giuseppina Molinaro; Flavia Fontana; Rubén Pareja Tello; Shiqi Wang; Sandra López Cérda; Giulia Torrieri; Alexandra Correia; Eero Waris; Jouni T. Hirvonen; Goncalo Barreto; Hélder A. Santos
• Keywords: Tannic acid; Biocompatibility; Proinflammatory cytokine; Nanobiotechnology

Tendinitis is a tendon disorder related to inflammation and pain, due an injury or overuse of the tissue, which hypocellular hypovascular, leading limited repair occurs in disorganized deposition extracellular matrix that leads scar formation fibrosis, ultimately resulting impaired integrity. Current conventional treatments are often ineffective, highlighting need for new therapeutic strategies. In this work, acetalated-dextran nanoparticles (AcDEX NPs) loaded with curcumin coated tannic acid (TA) developed exploit anti-inflammatory anti-fibrotic properties two compounds. For purpose, microfluidic technique was used order obtain particles precise size distribution, aiming decrease batch-to-batch variability possible future clinical translation. Coating TA increased not only stability nanosystem different media but also enhanced interaction cell-uptake primary human tenocytes KG-1 macrophages. The exhibited good biocompatibility toward these cell types release profile inflammatory environment. efficacy demonstrated by real-time quantitative polymerase chain reaction, showed decreasing expression NF-κb TA-coated NPs showing effect, gene TGF-β. Overall, loading AcDEX NPs, their synergistic activity, has promising application tendinitis.