Encapsulating Antibiotic and Protein-Stabilized Nanosilver into Sandwich-Structured Electrospun Nanofibrous Scaffolds for MRSA-Infected Wound Treatment

• DOI: 10.1021/acsami.3c10994
• Publication Date: 2023-10-10T20:38:06Z
• Authors: Ling Cai; Li Zhang; Jing Yang; Xinyi Zhu; Wei Wei; Minghui Ji; Huijun Jiang; Jin Chen
• Keywords: Biocompatibility; Electrospinning

With the increasing prevalence of microbial infections, which results in prolonged inflammation and delayed wound healing, development effective safe antimicrobial dressings multiple properties remains challenging for public health. Despite their various formats, available developed with limited functions may not fulfill diverse demands involved complex healing process. In this study, multifunctional sandwich-structured electrospinning nanofiber membranes (ENMs) were fabricated. According to structural composition, obtained ENMs included a hydrophilic inner layer loaded curcumin gentamicin sulfate, an antibacterial middle consisting bovine serum albumin stabilized silver oxide nanoparticles, hydrophobic outer layer. The prepared (SNM) exhibited good biocompatibility killing efficacy on Escherichia coli, Staphylococcus aureus, Methicillin-resistant S. aureus (MRSA). particular, transcriptomic analysis revealed that SNM inactivated MRSA by inhibiting its carbohydrate energy metabolism reduced bacterial resistance downregulating mecA. animal experiment, showed improved efficiency reducing load inflammation. Moreover, 16S rDNA sequencing indicated treatment accelerate without observed influence normal skin flora. Therefore, constructed promising potential as deal infected management.