Spatiotemporal Modulated Scaffold for Endogenous Bone Regeneration via Harnessing Sequentially Released Guiding Signals

• DOI: 10.1021/acsami.3c13963
• Publication Date: 2023-12-07T08:20:56Z
• Authors: Xinyu Wang; Wenli Dai; Chenyuan Gao; Liwen Zhang; Zhuo Wan; Tianyun Zhang; Yue Wang; Yujing Tang; Yingjie Yu; Xiaoping Yang; Qing Cai
• Keywords: Chondrogenesis; Endochondral ossification; Gelatin

The design of a scaffold that can regulate the sequential differentiation bone marrow mesenchymal stromal cells (BMSCs) according to endochondral ossification (ECO) mechanism is highly desirable for effective regeneration. In this study, we successfully fabricated dual-networked composite hydrogel composed gelatin and hyaluronic acid (termed GCDH-M), which sequentially release chondroitin sulfate (CS) magnesium/silicon (Mg/Si) ions provide spatiotemporal guidance chondrogenesis, angiogenesis, osteogenesis. fast CS from GCDH hydrogel, sustained releases Mg/Si are poly(lactide-co-glycolide) microspheres embedded in hydrogel. There difference rates between ions, resulting ability ions. dual networks modified via covalent bonding host-guest interactions render with some dynamic feature meet development BMSCs laden inside i.e., transforming into chondrogenic phenotype, further hypertrophic phenotype eventually an osteogenic phenotype. As evidenced by results vitro vivo evaluations, GCDH-M was proved be able create optimal microenvironment responding guiding signals, aligns rhythm ECO process ultimately boosts promising outcome achieved innovative system sheds light on novel targeting tissue engineering.