Orally Deliverable Sequence-Targeted Fucoxanthin-Loaded Biomimetic Extracellular Vesicles for Alleviation of Nonalcoholic Fatty Liver Disease

• DOI: 10.1021/acsami.3c18029
• Publication Date: 2024-02-20T12:40:11Z
• Authors: Caiyun Wu; Siyuan Xiang; Haitao Wang; Xiumin Zhang; Xueying Tian; Mingqian Tan; Wentao Su
• Keywords: Lipogenesis; Biocompatibility

Extracellular vesicles (EVs) possess favorable biocompatibility and immunological characteristics, making them optimal carriers for bioactive substances. In this study, an innovative hepatic-targeted vesicle system encapsulating with fucoxanthin (GA-LpEVs-FX) was successfully designed used to alleviate nonalcoholic fatty liver disease. The formulation entails the self-assembly of EVs derived from Lactobacillus paracasei (LpEVs), modification glycyrrhetinic acid (GA) via amide reaction offering liver-targeting capacity loading (FX) through sonication treatment. vitro experiments demonstrated that GA-LpEVs-FX effectively mitigated hepatic lipid accumulation attenuated reactive oxygen species-induced damage resulting (p < 0.05). vivo, exhibited significant downregulation lipogenesis-related proteins, namely, synthase (FAS), acetyl-CoA carboxylase (ACC1), sterol regulatory element binding protein 1 (SREBP-1), subsequently ameliorating metabolism disorders 0.05), stability significantly improved compared free FX. These findings establish a novel utilizing foodborne components disease alleviation.