Polymer Micropatches as B-Cell Engagers

• DOI: 10.1021/acsami.4c04385
• Publication Date: 2024-05-21T09:34:00Z
• Authors: Supriya Prakash; Ninad Kumbhojkar; Alexander P. Gott...; Kyung-Soo Park; Neha Kapate; Samir Mitragotri
• Keywords: Adoptive Cell Transfer; Naive B cell

B cells, despite their several unique functionalities, remain largely untapped for use as an adoptive cell therapy and are limited to in vitro antibody production. cells can be easily sourced, they possess excellent lymphoid-homing capabilities, act antigen-presenting (APCs), offering alternative dendritic (DCs), which have shown efficacy the clinical setting. Soluble factors such IL-4 anti-CD40 enhance activation, survival, capabilities of cells; however, it is difficult attain sufficiently high concentrations these biologics stimulate vivo. Micropatches Cell Engagers (MACE) polymeric microparticles, surface functionalized with anti-IgM, attach simultaneously engage multiple B-cell receptors (BCR) CD40 receptors. Stimulation through MACE, unlike free antibodies, enhanced display costimulatory molecules on surface, increased viability, improved antigen presentation by T vitro. activation MACE further synergized soluble anti-CD40. also elicited T-cell chemokine secretion cells. Upon intravenous transfer, MACE-bound homed spleen lymph nodes, key sites Adoptive transfer MACE-B pulsed CD4+ CD8+ epitopes ovalbumin significantly delayed tumor progression a murine subcutaneous EG7-OVA model, demonstrating functional benefit conferred MACE.