Regulation of Osteogenic Differentiation of hBMSCs by the Overlay Angles of Bone Lamellae-like Matrices
DOI:
10.1021/acsami.4c12847
Publication Date:
2024-10-11T03:02:27Z
AUTHORS (6)
ABSTRACT
Oriented fibers in bone lamellae are recognized for their contribution to the anisotropic mechanical performance of cortical bone. While increasing evidence highlights that such oriented also exhibit osteogenic induction preosteoblasts, little is known about effect overlay angle between on differentiation marrow-derived mesenchymal stem cells (BMSCs). In this study, lamellae-like fibrous matrices composed aligned core–shell [core: polycaprolactone (PCL)/type I collagen (Col I) + shell: Col I] nanofibers were seeded with human BMSCs (hBMSCs) and then laid over each other layer-by-layer (L-b-L) at selected angles (0 or 45°) form three-dimensional (3D) constructs. Upon culture 7 14 days, hBMSCs mineralization within assembly (LA) characterized by real-time PCR, Western blot, immunofluorescent staining markers, alizarin red calcium deposition. Compared those random (LA-RF) 45° (LA-AF-45), LA a 0° (LA-AF-0) exhibited noticeably higher hBMSCs, i.e., elevated gene expression OPN, OCN, RUNX2 protein levels ALP RUNX2, while promoting mineral deposition as indicated testing. Further analyses LA-AF-0 revealed an increase both total phosphorylated integrin β1, which subsequently increased focal adhesion kinase (FAK), FAK (p-FAK), extracellular signal ERK1/2 (p-ERK1/2). Inhibition β1 activity effectively reduced LA-AF-0-induced upregulation p-FAK markers (OPN, RUNX2), confirming involvement β1-FAK-ERK1/2 signaling. Altogether, nanofiber membranes regulates via signaling, unveiling effects tissue formation.
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