DNA Origami Barcodes for Immunostaining

• DOI: 10.1021/acsami.4c19153
• Publication Date: 2025-02-27T23:59:36Z
• Authors: Praneetha Sundar ...; Foram M. Joshi; Eric Vogelsberg; Glenn A. O. Cremers; Fatih N. Gür; Yusuke Sato; Tom F. A. de Greef; Marius Ader; Thomas Kurth; Diana Patricia Nu...; Thorsten L. Schmidt
• Keywords: DNA origami; Immunostaining

In histology, immunostaining of biological samples is a gold standard for studying cellular processes, such as the expression cell surface markers or uptake proteins and drug molecules. Immuno-gold labeling commonly used technique to achieve nanometer spatial resolution, but simultaneous visualization multiple antigens in parallel an unresolved challenge. Herein, we demonstrate DNA nanotechnology-based approach label transmission electron microscopy images tissue sections with high contrast patterns. For this, attached nanoparticles designated binding positions on origami structures that act visual "barcodes." These barcodes are then hybridized complementary strands DNA-modified antibodies bound their respective ultrathin resin sections. As proof concept, several types two different antibody techniques will expand multiplexing abilities highly modular way.