Functionalized Collagen Scaffold Neutralizing the Myelin-Inhibitory Molecules Promoted Neurites Outgrowth in Vitro and Facilitated Spinal Cord Regeneration in Vivo

• DOI: 10.1021/acsami.5b03879
• Publication Date: 2015-06-02T14:30:08Z
• Authors: Xing Li; Jin Han; Yannan Zhao; Wenyong Ding; Jianshu Wei; Sufang Han; Xianping Shang; Bin Wang; Bing Chen; Zhifeng Xiao; Jianwu Dai
• Keywords: Neurite; Dorsal root ganglion

Research has demonstrated that many myelin-associated inhibitory molecules jointly contribute to the failure of adult spinal cord regeneration. Therapies comprehensively targeting multiple nature injured are being concerned. Here, two collagen-binding proteins, CBD-EphA4LBD and CBD-PlexinB1LBD, were constructed, respectively, neutralize axon guidance ephrinB3 sema4D inhibit regeneration nerve fibers. The neutralizing proteins have proven their ability specifically bind collagen continuously release from scaffolds. They could also promote neurites outgrowth cerebellar granular neurons dorsal root ganglion in vitro. Subsequently, functionalized scaffolds by physically absorbing NEP1-40 immobilizing CBD-PlexinB1LBD transplanted into a rat T10 complete transection model. Our results showed rats received treatment transplanting scaffold exhibited great advantage on axonal locomotion recovery after injury.